Membrane-damaging action of alveolysin from Bacillus alvei
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چکیده
منابع مشابه
Generation of leukotrienes from human granulocytes by alveolysin from Bacillus alvei.
We investigated the effect of alveolysin on human granulocytes. Alveolysin is an exoprotein produced by Bacillus alvei and belongs to the group of sulfhydryl-activated cytolysins. Other members of this group are streptolysin O and theta-toxin from Clostridium perfringens. It is demonstrated that alveolysin leads to leukotriene generation from human granulocytes, which exert chemotactic (leukotr...
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Tryptophanase from Bacillus alvei exhibited the expected spectrum of pyridoxal-5'-phosphate-dependent reactions. It exhibited l-serine dehydratase, S-alkyl-cysteine lyase, and cysteine desulfhydrase activities, as well as the classic tryptophanase reactions (all beta elimination reactions). It also acted as a tryptophan synthetase (beta replacement reactions) using indole plus l-serine or l-cys...
متن کاملNonmotile Variants of Bacillus alvei.
The ability of Bacillus alvei to form migrating colonies upon the surfaces of solid media free from excessive moisture was reported previously by Smith and Clark (1938).2 A similar property has been noted by Roberts (1935) for Bacillus rotans. Recently Shinn (1938), in a cinematographic study of colony motility of B. alvei, remarked that it was surprising that such motility had been so long ove...
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Membrane-damaging toxins are thought to be responsible for the explosive clinical course of Bacillus endophthalmitis. This study analyzed the contribution of phosphatidylinositol-specific phospholipase C (PI-PLC) and phosphatidylcholine-specific phospholipase C (PC-PLC) to the pathogenesis of experimental Bacillus endophthalmitis. Isogenic mutants were constructed by insertion of lacZ into Baci...
متن کاملSelective purification by thiol-disulfide interchange chromatography of alveolysin, a sulfhydryl-activated toxin of Bacillus alvei. Toxin properties and interaction with cholesterol and liposomes.
Alveolysin has been purified in milligram quantities to apparent immunochemical and electrophoretic homogeneity from the supernatant culture fluid of Bacillus alvei. Purification involved ultrafiltration with concomitant molecular sieving on Amicon hollow fibers, thiol-disulfide interchange chromatography on thiopropyl-Sepharose 6B, and gel filtration on Sephacryl S-200 and Bio-Gel P-100. The p...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1981
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.32.3.1187-1192.1981